Open AccessFusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics
Author(s) -
Kumar Yashwant,
Zhang Limin,
Panigrahi Priyabrata,
Dholakia Bhushan B.,
Dewangan Veena,
Chavan Sachin G.,
Kunjir Shrikant M.,
Wu Xiangyu,
Li Ning,
Rajmohanan Pattuparambil R.,
Kadoo Narendra Y.,
Giri Ashok P.,
Tang Huiru,
Gupta Vidya S.
Publication year - 2016
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12522
Subject(s) - metabolomics , biology , fusarium oxysporum , proteomics , metabolic pathway , quantitative proteomics , fusarium , inoculation , transcriptome , metabolism , secondary metabolism , botany , gene , microbiology and biotechnology , gene expression , genetics , biochemistry , biosynthesis , bioinformatics , horticulture
Summary Molecular changes elicited by plants in response to fungal attack and how this affects plant–pathogen interaction, including susceptibility or resistance, remain elusive. We studied the dynamics in root metabolism during compatible and incompatible interactions between chickpea and Fusarium oxysporum f. sp. ciceri (Foc), using quantitative label‐free proteomics and NMR ‐based metabolomics. Results demonstrated differential expression of proteins and metabolites upon Foc inoculations in the resistant plants compared with the susceptible ones. Additionally, expression analysis of candidate genes supported the proteomic and metabolic variations in the chickpea roots upon Foc inoculation. In particular, we found that the resistant plants revealed significant increase in the carbon and nitrogen metabolism; generation of reactive oxygen species ( ROS ), lignification and phytoalexins. The levels of some of the pathogenesis‐related proteins were significantly higher upon Foc inoculation in the resistant plant. Interestingly, results also exhibited the crucial role of altered Yang cycle, which contributed in different methylation reactions and unfolded protein response in the chickpea roots against Foc. Overall, the observed modulations in the metabolic flux as outcome of several orchestrated molecular events are determinant of plant's role in chickpea–Foc interactions.