Open AccessOverexpression of patatin‐related phospholipase AIII δ altered plant growth and increased seed oil content in camelina
Author(s) -
Li Maoyin,
Wei Fang,
Tawfall Amanda,
Tang Michelle,
Saettele Allison,
Wang Xuemin
Publication year - 2015
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12304
Subject(s) - camelina , camelina sativa , biology , brassicaceae , canola , phosphatidylethanolamine , phosphatidylcholine , crop , botany , agronomy , food science , biochemistry , phospholipid , membrane
Summary Camelina sativa is a Brassicaceae oilseed species being explored as a biofuel and industrial oil crop. A growing number of studies have indicated that the turnover of phosphatidylcholine plays an important role in the synthesis and modification of triacylglycerols. This study manipulated the expression of a patatin‐related phospholipase AIIIδ ( pPLAIII δ) in camelina to determine its effect on seed oil content and plant growth. Constitutive overexpression of pPLAIII δ under the control of the constitutive cauliflower mosaic 35S promoter resulted in a significant increase in seed oil content and a decrease in cellulose content. In addition, the content of major membrane phospholipids, phosphatidylcholine and phosphatidylethanolamine, in 35S:: pPLAIII δ plants was increased. However, these changes in 35S:: pPLAIII δ camelina were associated with shorter cell length, leaves, stems, and seed pods and a decrease in overall seed production. When pPLAIII δ was expressed under the control of the seed specific, β‐conglycinin promoter, the seed oil content was increased without compromising plant growth. The results suggest that pPLAIII δ alters the carbon partitioning by decreasing cellulose content and increasing oil content in camelina.