Open AccessRNA‐guided transcriptional regulation in planta via synthetic dC as9‐based transcription factors
Author(s) -
Piatek Agnieszka,
Ali Zahir,
Baazim Hatoon,
Li Lixin,
Abulfaraj Aala,
AlShareef Sahar,
Aouida Mustapha,
Mahfouz Magdy M.
Publication year - 2015
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12284
Subject(s) - biology , crispr , repressor , cas9 , transcription (linguistics) , effector , transcriptional regulation , crispr interference , genetics , regulation of gene expression , trans activating crrna , transcription factor , gene , computational biology , microbiology and biotechnology , linguistics , philosophy
Summary Targeted genomic regulation is a powerful approach to accelerate trait discovery and development in agricultural biotechnology. Bacteria and archaea use clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR‐associated (Cas) regulatory systems for adaptive molecular immunity against foreign nucleic acids introduced by invading phages and conjugative plasmids. The type II CRISPR/Cas system has been adapted for genome editing in many cell types and organisms. A recent study used the catalytically inactive Cas9 ( dC as9) protein combined with guide‐RNAs ( gRNA s) as a DNA‐targeting platform to modulate gene expression in bacterial, yeast, and human cells. Here, we modified this DNA‐targeting platform for targeted transcriptional regulation in planta by developing chimeric dC as9‐based transcriptional activators and repressors. To generate transcriptional activators, we fused the dC as9 C‐terminus with the activation domains of EDLL and TAL effectors. To generate a transcriptional repressor, we fused the dC as9 C‐terminus with the SRDX repression domain. Our data demonstrate that dC as9 fusion with the EDLL activation domain ( dC as9:EDLL) and the TAL activation domain ( dC as9:TAD), guided by gRNA s complementary to selected promoter elements, induce strong transcriptional activation on Bs3::uidA targets in plant cells. Further, the dC as9:SRDX‐mediated transcriptional repression of an endogenous gene. Thus, our results suggest that the synthetic transcriptional repressor ( dC as9:SRDX) and activators ( dC as9:EDLL and dC as9:TAD) can be used as endogenous transcription factors to repress or activate transcription of an endogenous genomic target. Our data indicate that the CRISPR/ dC as9 DNA‐targeting platform can be used in plants as a functional genomics tool and for biotechnological applications.