English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Summary  When cultivated under stress conditions, many plants and algae accumulate oil. The unicellular green microalga   C hlamydomonas reinhardtii  accumulates neutral lipids (triacylglycerols;  TAG s) during nutrient stress conditions. Temporal changes in  TAG  levels in nitrogen (N)‐ and phosphorus (P)‐starved cells were examined to compare the effects of nutrient depletion on  TAG  accumulation in  C. reinhardtii .  TAG  accumulation and fatty acid composition were substantially changed depending on the cultivation stage before nutrient starvation. Profiles of  TAG  accumulation also differed between N and P starvation. Logarithmic‐growth‐phase cells diluted into fresh medium showed substantial  TAG  accumulation with both N and P deprivation. N deprivation induced formation of oil droplets concomitant with the breakdown of thylakoid membranes. In contrast, P deprivation substantially induced accumulation of oil droplets in the cytosol and maintaining thylakoid membranes. As a consequence, P limitation accumulated more  TAG  both per cell and per culture medium under these conditions. To enhance oil accumulation under P deprivation, we constructed a P deprivation‐dependent overexpressor of a   C hlamydomonas  type‐2 diacylglycerol acyl‐CoA acyltransferase ( DGTT 4) using a sulphoquinovosyldiacylglycerol 2 (  SQD 2 ) promoter, which was up‐regulated during P starvation. The transformant strongly enhanced  TAG  accumulation with a slight increase in 18 : 1 content, which is a preferred substrate of  DGTT 4. These results demonstrated enhanced  TAG  accumulation using a P starvation–inducible promoter.

plant biotechnology journal

Enhancement of extraplastidic oil synthesis in   C hlamydomonas reinhardtii  using a type‐2 diacylglycerol acyltransferase with a phosphorus starvation–inducible promoter