Open AccessA dual gene‐silencing vector system for monocot and dicot plants
Author(s) -
Liou MingRu,
Huang YingWen,
Hu ChungChi,
Lin NaSheng,
Hsu YauHeiu
Publication year - 2014
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12140
Subject(s) - biology , nicotiana benthamiana , gene silencing , potexvirus , functional genomics , rna interference , gene , brachypodium distachyon , rna silencing , genetically modified crops , transgene , genetics , rna , genome , genomics , coat protein
Summary Plant virus‐based gene‐silencing vectors have been extensively and successfully used to elucidate functional genomics in plants. However, only limited virus‐induced gene‐silencing (VIGS) vectors can be used in both monocot and dicot plants. Here, we established a dual gene‐silencing vector system based on Bamboo mosaic virus (BaMV) and its satellite RNA (satBaMV). Both BaMV and satBaMV vectors could effectively silence endogenous genes in Nicotiana benthamiana and Brachypodium distachyon . The satBaMV vector could also silence the green fluorescent protein (GFP) transgene in GFP transgenic N. benthamiana . GFP transgenic plants co‐agro‐inoculated with BaMV and satBaMV vectors carrying sulphur and GFP genes, respectively, could simultaneously silence both genes. Moreover, the silenced plants could still survive with the silencing of genes essential for plant development such as heat‐shock protein 90 (Hsp90) and Hsp70 . In addition, the satBaMV‐ but not BaMV‐based vector could enhance gene‐silencing efficiency in newly emerging leaves of N. benthamiana deficient in RNA‐dependant RNA polymerase 6. The dual gene‐silencing vector system of BaMV and satBaMV provides a novel tool for comparative functional studies in monocot and dicot plants.