Open AccessA novel membrane‐bound E 3 ubiquitin ligase enhances the thermal resistance in plants
Author(s) -
Liu ZhiBin,
Wang JianMei,
Yang FengXi,
Yang Liang,
Yue YuFei,
Xiang JunBei,
Gao Mei,
Xiong FangJian,
Lv Dong,
Wu XianJun,
Liu Ning,
Zhang Xun,
Li XuFeng,
Yang Yi
Publication year - 2014
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12120
Subject(s) - biology , ubiquitin ligase , heat shock protein , microbiology and biotechnology , brassica , ubiquitin , heat shock , hsp90 , oryza sativa , gene , botany , biochemistry
Summary High temperature stress disturbs cellular homoeostasis and results in a severe retardation in crop growth and development. Thus, it is important to reveal the mechanism of plants coping with heat stress. In this study, a novel gene that we identified from B rassica napus , referred to as B n TR 1 , was found to play a key role in heat stress response in planta . B n TR 1 is a membrane‐bound RING v ( C 4 HC 3 ) protein that displays E 3 ligase activity in vitro . We demonstrated that modest expression of B n TR 1 is sufficient to minimize adverse environmental influence and confers thermal resistance on development without any detrimental effects in B . napus and O ryza sativa . Our investigation into the action mechanism indicates that B n TR 1 is likely to be involved in mediating Ca 2+ dynamics by regulating the activity of calcium channels, which further alters the transcripts of heat shock factors and heat shock proteins contributing to plant thermotolerance. Hence, our study identified B n TR 1 as a novel key factor underlying a conserved mechanism conferring thermal resistance in plants.