Open AccessCamelina seed transcriptome: a tool for meal and oil improvement and translational research
Author(s) -
Nguyen Huu T.,
Silva Jillian E.,
Podicheti Ram,
Macrander Jason,
Yang Wenyu,
Nazarenus Tara J.,
Nam JeongWon,
Jaworski Jan G.,
Lu Chaofu,
Scheffler Brian E.,
Mockaitis Keithanne,
Cahoon Edgar B.
Publication year - 2013
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12068
Subject(s) - camelina sativa , camelina , biology , transcriptome , arabidopsis , oleic acid , arabidopsis thaliana , gene , microbiology and biotechnology , food science , genetics , botany , crop , gene expression , agronomy , mutant
Summary Camelina ( C amelina sativa ), a B rassicaceae oilseed, has received recent interest as a biofuel crop and production platform for industrial oils. Limiting wider production of camelina for these uses is the need to improve the quality and content of the seed protein‐rich meal and oil, which is enriched in oxidatively unstable polyunsaturated fatty acids that are deleterious for biodiesel. To identify candidate genes for meal and oil quality improvement, a transcriptome reference was built from 2047 S anger EST s and more than 2 million 454‐derived sequence reads, representing genes expressed in developing camelina seeds. The transcriptome of approximately 60K transcripts from 22 597 putative genes includes camelina homologues of nearly all known seed‐expressed genes, suggesting a high level of completeness and usefulness of the reference. These sequences included candidates for 12 S (cruciferins) and 2 S (napins) seed storage proteins ( SSP s) and nearly all known lipid genes, which have been compiled into an accessible database. To demonstrate the utility of the transcriptome for seed quality modification, seed‐specific RNA i lines deficient in napins were generated by targeting 2 S SSP genes, and high oleic acid oil lines were obtained by targeting FATTY ACID DESATURASE 2 ( FAD 2 ) and FATTY ACID ELONGASE 1 ( FAE 1 ). The high sequence identity between A rabidopsis thaliana and camelina genes was also exploited to engineer high oleic lines by RNA i with A rabidopsis FAD 2 and FAE 1 sequences. It is expected that these transcriptomic data will be useful for breeding and engineering of additional camelina seed traits and for translating findings from the model A rabidopsis to an oilseed crop.