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Relationship Between ABCB 1 Polymorphisms and Cold Pain Sensitivity Among Healthy Opioid‐naive Malay Males
Author(s) -
Zahari Zalina,
Lee Chee Siong,
Ibrahim Muslih Abdulkarim,
Musa Nurfadhlina,
Mohd Yasin Mohd Azhar,
Lee Yeong Yeh,
Tan Soo Choon,
Mohamad Nasir,
Ismail Rusli
Publication year - 2017
Publication title -
pain practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 58
eISSN - 1533-2500
pISSN - 1530-7085
DOI - 10.1111/papr.12546
Subject(s) - pain tolerance , medicine , threshold of pain , low back pain , analgesic , gastroenterology , traditional medicine , anesthesia , pharmacology , pathology , alternative medicine
Background Endogenous and exogenous opioids are substrates of the permeability glycoprotein (P‐gp) efflux transporter, which is encoded by the ABCB 1 ( MDR 1 ) gene. Genetic polymorphisms of ABCB 1 may contribute to interindividual differences in pain modulation and analgesic responses. We investigated the relationship between ABCB 1 polymorphisms and cold pain sensitivity among healthy males. Methods Cold pain responses, including pain threshold and pain tolerance, were measured using the cold‐pressor test ( CPT ). DNA was extracted from whole blood and genotyped for ABCB 1 polymorphisms, including c.1236C>T (rs1128503), c.2677G>T/A (rs2032582), and c.3435C>T (rs1045642), using the allelic discrimination real‐time polymerase chain reaction. Results A total of 152 participants were recruited in this observational study. Frequencies of mutated allele for c.1236C>T, c.2677G>T/A, and c.3435C>T polymorphisms were 56.6%, 49.7%, and 43.4%, respectively. Our results revealed an association of the CGC / CGC diplotype (c.1236C>T, c.2677G>T/A, and c.3435C>T) with cold pain sensitivity. Participants with the CGC / CGC diplotype had 90% and 72% higher cold pain thresholds (87.62 seconds vs. 46.19 seconds, P = 0.010) and cold pain tolerances (97.24 seconds vs. 56.54 seconds, P = 0.021), respectively, when compared with those without the diplotype. Conclusion The CGC / CGC diplotype of ABCB 1 polymorphisms was associated with variability in cold pain threshold and pain tolerance in healthy males.