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Decrease in antigen‐specific CD 63 basophil expression is associated with the development of tolerance to egg by SOTI in children
Author(s) -
Vila Leticia,
Moreno Ana,
Gamboa Pedro M.,
MartínezAranguren Rubén,
Sanz Maria L.
Publication year - 2013
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/pai.12070
Subject(s) - medicine , ovalbumin , basophil activation , basophil , egg white , immunology , population , andrology , antigen , immunoglobulin e , biology , food science , antibody , environmental health
Background In the last decade, there have been an increasing number of studies on achieving tolerance to foods by specific oral tolerance induction ( SOTI ). Still, the underlying mechanism of SOTI is unknown. Our aim was to describe changes in CD 63 expression on basophils following in vitro Ag‐specific stimulation by basophil activation test ( BAT ), after SOTI with egg in a pediatric population. Methods Ten children with persistent allergy to egg were included. Skin prick tests ( SPTs ) and open food challenges ( OFCs ) were performed before SOTI . Specific IgE determination and BAT with egg white ( EW ), ovomucoid ( OM ), and ovalbumin ( OVA ) were performed before and after 1 month of the buildup phase of SOTI . Results Total tolerance to egg was achieved in 9 cases and partial in one. After SOTI , there was a significant decrease in mean specific IgE levels (p < 0.05). CD 63 expression also decreased (p < 0.05) in all patients. Conclusion Decrease in Ag‐specific basophil responsiveness is associated with the development of clinical tolerance by SOTI .