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Children monosensitized to pine nuts have similar patterns of sensitization
Author(s) -
Novembre Elio,
Mori Francesca,
Barni Simona,
Ferrante Giuliana,
Pucci Neri,
Ballabio Cinzia,
Uberti Francesca,
Penas Elena,
Restani Patrizia
Publication year - 2012
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/pai.12012
Subject(s) - nut , medicine , allergy , allergen , sensitization , brazil nut , gel electrophoresis , anaphylaxis , sodium dodecyl sulfate , food science , immunology , biology , biochemistry , structural engineering , engineering
Background Several cases of pine nut allergies and anaphylaxis have been reported in the literature, but only few pine nut allergens have been characterized. The aim of this research is to identify through immunoelectrophoretic techniques the major pine nut allergens in a group of children monosensitized to pine nuts. Methods We studied five children with pine nut allergies and no other sensitization to food except to pine nuts, confirmed by in vivo (prick test, prick‐to‐prick) and in vitro tests (specific I g E determinations [ CAP ‐ FEIA ]). The protein profile of pine nuts was analyzed by Sodium Dodecyl sulfate Polyacrylamide Gel Electrophoresis ( SDS ‐ PAGE ). Immunoblotting was performed after incubation of membranes with the sera from the children included in the present study. Results Immunoblotting ( SDS ‐ PAGE ) demonstrated five similar bands between 6 and 47 kDa in all the subjects studied. Conclusion These bands should be considered the potential allergens for pine nut allergic children.