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Is it possible to contaminate the temporomandibular joint by arthrocentesis?
Author(s) -
OlsenBergem H.,
Kristoffersen A.K.,
Bjørnland T.,
Aas J.A.
Publication year - 2018
Publication title -
oral surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.156
H-Index - 11
eISSN - 1752-248X
pISSN - 1752-2471
DOI - 10.1111/ors.12331
Subject(s) - arthrocentesis , medicine , temporomandibular joint , synovial fluid , arthritis , contamination , dentistry , pathology , osteoarthritis , biology , ecology , alternative medicine
Bacterial contamination of the temporomandibular joint ( TMJ ) by needle penetration of the skin has previously been discussed as a contributing factor in joint arthritis. The purpose of this study was to investigate the presence of bacterial deoxyribonucleic acid ( DNA ) in synovial fluid, and to detect any possible iatrogenic contamination during arthrocentesis of the TMJ in patients with juvenile idiopathic arthritis and rheumatoid arthritis. Method Synovial fluid ( SF ) and skin swab samples (before and after disinfection) were collected from 54 TMJ s in 30 patients with TMJ arthritis. Bacterial detection and classification was performed using 16S rRNA pyrosequencing. Results Bacterial DNA was detected in 31 joints (57%) in 19 patients (63%). In six 12 joints (20%) in six patients specific bacterial species were detected in both skin samples and in the TMJ , indicating a possible contamination. 22 different bacterial species were detected in synovial fluid from these six patients. Conclusion Bacterial DNA in TMJ SF with contamination was detected in 20% of the patients. Studies are needed to evaluate the consequences of potential contamination with bacterial DNA in SF with regard to arthrocentesis and treatment of TMJ arthritis.