Premium
Inflammatory response of uric acid produced by Porphyromonas gingivalis gingipains
Author(s) -
Jun HyeKyung,
An SunJin,
Kim Hyun Young,
Choi BongKyu
Publication year - 2020
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/omi.12309
Subject(s) - porphyromonas gingivalis , uric acid , proinflammatory cytokine , proteases , xanthine , chemistry , inflammasome , microbiology and biotechnology , periodontitis , inflammation , biology , biochemistry , immunology , medicine , enzyme
Uric acid is a potential metabolite that serves as a danger‐associated molecular pattern (DAMP) and induces inflammatory responses in sterile environments. Porphyromonas gingivalis is a keystone periodontopathogen, and its gingipain proteases play a critical role in the pathogenesis of periodontitis. In this study, we demonstrate that P. gingivalis gingipains play a role in THP‐1 macrophage uric acid production by increasing the expression and activity of xanthine oxidoreductase (XOR). Uric acid sodium salt induces caspase‐1 activation, cell death, and the expression of proinflammatory cytokines, including IL‐1α, IL‐6, and IL‐8, in the human keratinocyte HOK‐16B cell line. Our results suggest that gingipain‐induced uric acid can mediate inflammation in periodontal tissue cells.