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The contribution of Tannerella forsythia dipeptidyl aminopeptidase IV in the breakdown of collagen
Author(s) -
Yost Susan,
DuranPinedo Ana E.
Publication year - 2018
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/omi.12244
Subject(s) - tannerella forsythia , forsythia , periodontitis , serine protease , aminopeptidase , porphyromonas gingivalis , dipeptidyl peptidase , microbiology and biotechnology , chemistry , protease , biology , biochemistry , medicine , enzyme , amino acid , pathology , honeysuckle , leucine , alternative medicine , traditional chinese medicine
Summary In this study, we characterized a serine protease from Tannerella forsythia that degrades gelatin, type I, and III collagen. Tannerella forsythia is associated with periodontitis progression and severity. The primary goal of this research was to understand the mechanisms by which T. forsythia contributes to periodontitis progression. One of our previous metatranscriptomic analysis revealed that during periodontitis progression T. forsythia highly expressed the bfor_1659 ORF. The N‐terminal end is homologous to dipeptidyl aminopeptidase IV (DPP IV). DPP IV is a serine protease that cleaves X‐Pro or X‐Ala dipeptide from the N‐terminal end of proteins. Collagen type I is rich in X‐Pro and X‐Ala sequences, and it is the primary constituent of the periodontium. This work assessed the collagenolytic and gelatinolytic properties of BFOR_1659. To that end, the complete BFOR_1659 and its domains were purified as His‐tagged recombinant proteins, and their collagenolytic activity was tested on collagen‐like substrates, collagen type I and III combined, and on the extracellular matrix (ECM) formed on human gingival fibroblasts culture HGF‐1. BFOR_1659 was only found in T. forsythia supernatants, highlighting its potential role on the pathogenicity of T. forsythia . We also found that BFOR_1659 efficiently degrades all tested substrates but the individual domains were inactive. Given that BFOR_1659 is highly expressed in the periodontal pocket, its clinical relevance is suggested to periodontitis progression.