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Cultivation of Peptidiphaga gingivicola from subgingival plaque: The first representative of a novel genus of Actinomycetaceae
Author(s) -
Beall C.J.,
Mokrzan E.M.,
Griffen A.L.,
Leys E.J.
Publication year - 2018
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/omi.12205
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , microbiology and biotechnology , phylogenetics , bacteria , genus , biochemistry , genetics , gene , zoology
Summary A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA 112 T is a facultative Gram‐positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine and tyrosine, but does not appear to use carbohydrates. Urease, esculin, indole, catalase and nitrate reduction tests were all negative. Major cellular fatty acids were C 18:0 , C 12:0 , C 16:0 , C 18:1 w 9 c and C 20:0 . The genome was sequenced and is 2.4 Mbp in length and has 64% GC content. Based on phylogenetics of the 16S rRNA sequence and concatenated alignments of 37 conserved proteins, BA 112 T belongs to the family Actinomycetaceae but is located on a branch of the tree without currently named members. Based on our phenotypic and phylogenetic studies, we propose that BA 112 T is the first known representative of a new genus, for which the name Peptidiphaga gingivicola gen. nov., sp. nov. is proposed. The type strain is BA 112 T .