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Tannerella forsythia Gro EL induces inflammatory bone resorption and synergizes with interleukin‐17
Author(s) -
Jung Y.J.,
Choi Y.J.,
An S.J.,
Lee H.R.,
Jun H.K.,
Choi B.K.
Publication year - 2017
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/omi.12172
Subject(s) - tannerella forsythia , forsythia , prostaglandin e2 , bone resorption , interleukin 17 , interleukin , osteoprotegerin , immunology , cytokine , microbiology and biotechnology , medicine , biology , pathology , endocrinology , honeysuckle , alternative medicine , receptor , traditional chinese medicine , activator (genetics)
Summary Tannerella forsythia is a major periodontal pathogen, and T. forsythia Gro EL is a molecular chaperone homologous to human heat‐shock protein 60. Interleukin‐17 ( IL ‐17) has been implicated in the pathogenesis of periodontitis and several systemic diseases. This study investigated the potential of T. forsythia Gro EL to induce inflammatory bone resorption and examined the cooperative effect of IL ‐17 and T. forsythia Gro EL on inflammatory responses. Human gingival fibroblasts ( HGF s) and periodontal ligament ( PDL ) fibroblasts were stimulated with T. forsythia Gro EL and/or IL ‐17. Gene expression of IL ‐6, IL ‐8, and cyclooxygenase‐2 ( COX ‐2) and concentrations of IL ‐6, IL ‐8, and prostaglandin E 2 ( PGE 2 ) were measured by real‐time reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assays, respectively. After stimulation of MG 63 cells with T. forsythia Gro EL and/or IL ‐17, gene expression of osteoprotegerin ( OPG ) was examined. After subcutaneous injection of T. forsythia Gro EL and/or IL ‐17 above the calvaria of BALB /c mice, calvarial bone resorption was assessed by micro‐computed tomography and histological examination. Tannerella forsythia Gro EL induced IL ‐6 and IL ‐8 production in HGF s and PDL cells, and IL ‐17 further promoted IL ‐6 and IL ‐8 production. Both T. forsythia Gro EL and IL ‐17 synergistically increased PGE 2 production and inhibited OPG gene expression. Calvarial bone resorption was induced by T. forsythia Gro EL injection, and simultaneous injection of T. forsythia Gro EL and IL ‐17 further increased bone resorption. These results suggest that T. forsythia Gro EL is a novel virulence factor that can contribute to inflammatory bone resorption caused by T. forsythia and synergizes with IL ‐17 to exacerbate inflammation and bone resorption.