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Pathogenic potential of Tannerella forsythia enolase
Author(s) -
Lee JY.,
Jung YJ.,
Jun HK.,
Choi BK.
Publication year - 2016
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/omi.12115
Subject(s) - tannerella forsythia , forsythia , biology , microbiology and biotechnology , medicine , honeysuckle , alternative medicine , pathology , traditional chinese medicine
Summary Although enolases are cytosolic enzymes involved in the glycolytic pathway, they can also be secreted or expressed on the surface of a variety of eukaryotic cells and bacteria. Surface‐exposed enolases of eukaryotes and bacteria can function as plasminogen receptors. Furthermore, antibodies raised against bacterial enolases can react with host enolases, suggesting molecular mimicry between bacterial and host enzymes. In this study, we analyzed an enolase of the major periodontopathogen Tannerella forsythia , which is either secreted or present on the cell surface, via matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry and immunofluorescence, respectively. The T. forsythia enolase retained the enzymatic activity converting 2‐phosphoglycerate to phosphoenolpyruvate and showed plasminogen binding and activating ability, which resulted in the degradation of fibronectin secreted from human gingival fibroblasts. In addition, it induced proinflammatory cytokine production, including interleukin‐1β ( IL ‐1β), IL ‐6, IL ‐8, and tumour necrosis factor‐α (TNF‐a) in the human THP ‐1 monocytic cell line. Taken together, our results demonstrate that T. forsythia enolase plays a role in pathogenesis in the host by plasminogen activation and proinflammatory cytokine induction, which has the potential to exaggerate inflammation in periodontitis.