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Microbial dynamics during conversion from supragingival to subgingival biofilms in an in vitro model
Author(s) -
Thurnheer T.,
Bostanci N.,
Belibasakis G.N.
Publication year - 2016
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/omi.12108
Subject(s) - biofilm , fusobacterium nucleatum , treponema denticola , microbiology and biotechnology , streptococcus oralis , actinomyces naeslundii , veillonella , streptococcus mutans , tannerella forsythia , porphyromonas gingivalis , streptococcus mitis , biology , aggregatibacter actinomycetemcomitans , prevotella intermedia , dental plaque , anaerobic exercise , saliva , actinomyces , streptococcus gordonii , bacteria , streptococcus , medicine , biochemistry , honeysuckle , physiology , genetics , alternative medicine , traditional chinese medicine , pathology
Summary The development of dental caries and periodontal diseases result from distinct shifts in the microbiota of the tooth‐associated biofilm. This in vitro study aimed to investigate changes in biofilm composition and structure, during the shift from a ‘supragingival’ aerobic profile to a ‘subgingival’ anaerobic profile. Biofilms consisting of Actinomyces oris , Candida albicans , Fusobacterium nucleatum , Streptococcus oralis , Streptococcus mutans and Veillonella dispar were aerobically grown in saliva‐containing medium on hydroxyapatite disks. After 64 h, Campylobacter rectus , Prevotella intermedia and Streptococcus anginosus were further added along with human serum, while culture conditions were shifted to microaerophilic. After 96 h, Porphyromonas gingivalis , Tannerella forsythia and Treponema denticola were finally added and the biofilm was grown anaerobically for another 64 h. At the end of each phase, biofilms were harvested for species‐specific quantification and localization. Apart from C. albicans , all other species gradually increased during aerobic and microaerophilic conditions, but remained steady during anaerobic conditions. Biofilm thickness was doubled during the microaerophilic phase, but remained steady throughout the anaerobic phase. Extracellular polysaccharide presence was gradually reduced throughout the growth period. Biofilm viability was reduced during the microaerophilic conversion, but was recovered during the anaerobic phase. This in vitro study has characterized the dynamic structural shifts occurring in an oral biofilm model during the switch from aerobic to anaerobic conditions, potentially modeling the conversion of supragingival to subgingival biofilms. Within the limitations of this experimental model, the findings may provide novel insights into the ecology of oral biofilms.