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TRIB 3 mediates the expression of Wnt5a and activation of nuclear factor‐κB in Porphyromonas endodontalis lipopolysaccharide‐treated osteoblasts
Author(s) -
Yu Y.,
Qiu L.,
Guo J.,
Yang D.,
Qu L.,
Yu J.,
Zhan F.,
Xue M.,
Zhong M.
Publication year - 2015
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/omi.12094
Subject(s) - lipopolysaccharide , phosphorylation , iκbα , gene knockdown , nf κb , microbiology and biotechnology , biology , chemistry , signal transduction , biochemistry , gene , immunology
Summary Porphyromonas endodontalis lipopolysaccharide ( LPS ) is considered to be correlated with the progression of bone resorption in periodontal and periapical diseases. Wnt5a has recently been implicated in inflammatory processes, but its role is unclear as a P. endodontalis LPS ‐induced mediator in osteoblasts. Tribbles homolog 3 ( TRIB 3) encodes a pseudokinase and has been linked to inflammation in certain situations. Here, we found that P. endodontalis LPS induced Wnt5a expression in a dose‐ and time‐dependent manner and it also upregulated translocation, phosphorylation and transcriptional activity of nuclear factor‐κB ( NF ‐κB) in MC 3T3‐E1 cells. Bay 11‐7082 blocked the translocation of NF ‐κB and Wnt5a expression induced by P. endodontalis LPS . Chromatin immunoprecipitation assay further established that induction of Wnt5a by P. endodontalis LPS was mediated through the NF ‐κB p65 subunit. Additionally, P. endodontalis LPS increased expression of TRIB 3 in osteoblasts after 10 h simulated time. Overexpression of TRIB 3 enhanced NF ‐κB phosphorylation and Wnt5a induction, whereas knockdown of TRIB 3 inhibited NF ‐κB phosphorylation and Wnt5a expression in P. endodontalis LPS ‐stimulated osteoblasts. These results suggest that P. endodontalis LPS has the ability to promote the expression of Wnt5a in mouse osteoblasts, and this induction is mainly mediated by NF ‐κB pathway. TRIB 3 seems to modulate the sustained expression of Wnt5a in osteoblasts stimulated by P. endodontalis LPS , as well as regulating NF ‐κB phosphorylation.