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Metabolome variations in the P orphyromonas gingivalis vim A mutant during hydrogen peroxide‐induced oxidative stress
Author(s) -
McKenzie R.M.E.,
Aruni W.,
Johnson N.A.,
Robles A.,
Dou Y.,
Henry L.,
Boskovic D.S.,
Fletcher H.M.
Publication year - 2015
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/omi.12075
Subject(s) - hydrogen peroxide , mutant , oxidative stress , metabolome , chemistry , oxidative phosphorylation , biochemistry , microbiology and biotechnology , biology , gene , metabolite
Summary The adaptability and survival of P orphyromonas gingivalis in the oxidative microenvironment of the periodontal pocket are indispensable for survival and virulence, and are modulated by multiple systems. Among the various genes involved in P . gingivalis oxidative stress resistance, vim A gene is a part of the 6.15‐kb locus. To elucidate the role of a P . gingivalis vim A ‐defective mutant in oxidative stress resistance, we used a global approach to assess the transcriptional profile, to study the unique metabolome variations affecting survival and virulence in an environment typical of the periodontal pocket. A multilayered protection strategy against oxidative stress was noted in P . gingivalis FLL 92 with upregulation of detoxifying genes. The duration of oxidative stress was shown to differentially modulate transcription with 94 (87%) genes upregulated twofold during 10 min and 55 (83.3%) in 15 min. Most of the upregulated genes (55%), fell in the hypothetical/unknown/unassigned functional class. Metabolome variation showed reduction in fumarate and formaldehyde, hence resorting to alternative energy generation and maintenance of a reduced metabolic state. There was upregulation of transposases, genes encoding for the metal ion binding protein transport and secretion system.