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Comparative genomic hybridization and transcriptome analysis with a pan‐genome microarray reveal distinctions between JP 2 and non‐ JP 2 genotypes of A ggregatibacter actinomycetemcomitans
Author(s) -
Huang Y.,
Kittichotirat W.,
Mayer M.P.A.,
Hall R.,
Bumgarner R.,
Chen C.
Publication year - 2013
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/omi.12005
Subject(s) - biology , transcriptome , genotype , genetics , genome , virulence , operon , aggregatibacter actinomycetemcomitans , gene , dna microarray , microbiology and biotechnology , gene expression , bacteria , escherichia coli , porphyromonas gingivalis
Summary It was postulated that the highly virulent JP 2 genotype of A ggregatibacter actinomycetemcomitans may possess a constellation of distinct virulence determinants not found in non‐ JP 2 genotypes. This study compared the genome content and the transcriptome of the serotype b JP 2 genotype and the closely related serotype b non‐ JP 2 genotype of A . actinomycetemcomitans . A custom‐designed pan‐genomic microarray of A . actinomycetemcomitans was constructed and validated against a panel of 11 sequenced reference strains. The microarray was subsequently used for comparative genomic hybridization of serotype b strains of JP 2 (six strains) and non‐ JP 2 (six strains) genotypes, and for transcriptome analysis of strains of JP 2 (three strains) and non‐ JP 2 (two strains). Two JP 2‐specific and two non‐ JP 2‐specific genomic islands were identified. In one instance, distinct genomic islands were found to be inserted into the same locus among strains of different genotypes. Transcriptome analysis identified five operons, including the leukotoxin operon, to have at least two genes with an expression ratio of 2 or greater between genotypes. Two of the differentially expressed operons were members of the membrane‐bound nitrate reductase system ( nap operon) and the T ol‐ P al system of gram‐negative bacterial species. This study is the first to demonstrate the differences in the full genome content and gene expression between A . actinomycetemcomitans strains of JP 2 and non‐ JP 2 genotypes. The information is essential for designing hypothesis‐driven experiments to examine the pathogenic mechanisms of A . actinomycetemcomitans .