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Neuroinflammation changes with periodontal inflammation status during periodontitis in wild‐type mice
Author(s) -
Wang Xu,
Tong Yuxin,
Zhang Jiayu,
Khan Nazia,
Zhang Kaili,
Bai Huihui,
Zhang Qianqian,
Chen Yue
Publication year - 2021
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.13618
Subject(s) - astrogliosis , periodontitis , medicine , inflammation , neuroinflammation , dental alveolus , pathology , bone resorption , dentistry , central nervous system
Objective To investigate neuroinflammation under different periodontal status. Materials and Methods Experimental periodontitis was induced by molar ligation (Lig group) or periodontal injection of lipopolysaccharide (LPS, Lps group). Periodontal status was assessed by alveolar bone resorption and periodontal inflammation. Micro‐computed tomography and haematoxylin–eosin staining were performed to assess alveolar bone resorption and periodontal inflammation, respectively. Neuroinflammation was assessed by glial cell proliferation and proinflammatory factor expression. Microgliosis was determined by immunofluorescence. Astrogliosis was determined by immunohistochemistry. Expressions of tumour necrosis factor‐alpha (TNF‐α) and interleukin (IL)‐1β were assessed by enzyme‐linked immunosorbent assay. Results Microgliosis and astrogliosis in the Lig group were notable with molar ligation for 2 weeks and 4 weeks ( p < .05), but were only slightly different similar from the control group by week 12. Microgliosis and astrogliosis in the Lps group were significant with LPS injection for 4 and 8 weeks ( p < .05). The groups displayed a positive correlation between the degree of periodontal inflammation and the number of glial cells ( p < .05). Expressions of IL‐1β and TNF‐α in the Lps group were significantly increased with LPS injection for 8 weeks ( p < .05). In the Lig group, only TNF‐α was highly expressed with molar ligation for 12 weeks ( p < .05). Conclusion Both models demonstrated that the inflammatory response in the hippocampus of mice can change during periodontitis depending on the periodontal inflammation status.