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Deregulation of desmosomal proteins and extracellular matrix proteases in odontogenic keratocyst
Author(s) -
Diniz Marina Gonçalves,
DuarteAndrade Filipe Fideles,
Stussi Fernanda,
Vitório Jéssica Gardone,
Fonseca Felipe Paiva,
Ramos Domingues Romênia,
Paes Leme Adriana F.,
Gomes Carolina Cavaliéri,
Gomez Ricardo Santiago
Publication year - 2021
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.13598
Subject(s) - desmosome , keratocyst , extracellular matrix , proteases , proteomics , downregulation and upregulation , microbiology and biotechnology , keratin , biology , chemistry , pathology , biochemistry , cell , odontogenic , medicine , enzyme , gene
Objective Odontogenic keratocyst (OKC) is a benign lesion that tends to recur after surgical treatment. In an attempt to clarify the molecular basis underlining the OKC pathobiology, we aimed to analyze its proteomic profile. Materials and Methods We compared the proteomic profiles of five OKC and matched normal oral mucosa by using liquid chromatography–tandem mass spectrometry (LC‐MS/MS). Then, we performed enrichment analysis and a literature search for the immunoexpression of the proteomics targets. Results We identified 1,150 proteins and 72 differently expressed proteins (log2 fold change ≥ 1.5; p < .05). Twenty‐seven peptides were exclusively detected in the OKC samples. We found 35 enriched pathways related to cell differentiation and tissue architecture, including keratinocyte differentiation, keratinization, desmosome, and extracellular matrix (ECM) organization and degradation. The immunoexpression information of 11 out of 50 proteins identified in the enriched pathways was obtained. We found the downregulation of four desmosomal proteins ( JUP, PKP1 , PKP3 , and PPL ) and upregulation of ECM proteases (MMP‐2, MMP‐9, and cathepsins). Conclusions Proteomic analysis strengthened the notion that OKC cells have a similar proteomic profile to oral keratinocytes. Contextual investigation of the differentially expressed proteins revealed the deregulation of desmosome proteins and ECM degradation as important alterations in OKC pathobiology.