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miR‐146a‐5p attenuates IL‐1β‐induced IL‐6 and IL‐1β expression in a cementoblast‐derived cell line
Author(s) -
Pan Jiawen,
Du Mingyuan,
Cao Zhengguo,
Zhang Chen,
Hao Yunru,
Zhu Jiaqi,
He Hong
Publication year - 2020
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.13333
Subject(s) - cementoblast , p38 mitogen activated protein kinases , western blot , interleukin , mapk/erk pathway , signal transduction , transfection , microbiology and biotechnology , chemistry , cell culture , cytokine , immunology , biology , medicine , pathology , cementum , gene , biochemistry , genetics , dentin
Objective miR‐146a is widely induced during the immune response. However, little is known about the biogenesis, function and mechanism of miR‐146a in cementoblasts during the pathogenesis of periodontitis. This study aimed to investigate the effects of miR‐146a in murine cementoblast‐derived OCCM‐30 cells following IL‐1β stimulation. Materials and methods OCCM‐30 cells were cultured and exposed to IL‐1β. IL‐6, IL‐1β and TNF‐α, and miR‐146a‐5p expression was assessed by qRT‐PCR. Mimics/inhibitors were transiently transfected into cells to determine the function of miR‐146a‐5p. Signalling pathways including p38 MAPK, ERK1/2 and NF‐κB were studied by using specific inhibitors. The indicated proteins were measured by Western blot analysis and ELISA. Results In IL‐1β‐stimulated OCCM‐30 cells, the expression levels of miR‐146a‐5p along with IL‐6 and IL‐1β increased in a time‐dependent manner. The ERK1/2, p38 MAPK and NF‐κB pathway were activated upon IL‐1β stimulation. Blocking the NF‐κB pathway decreased IL‐6, IL‐1β and miR‐146a‐5p expression. The overexpression of miR‐146a‐5p reduced IL‐6 and IL‐1β expression, while the inhibition of miR‐146a‐5p increased IL‐6 and IL‐1β expression in IL‐1β‐treated OCCM‐30 cells. miR‐146a‐5p attenuated IL‐6 and IL‐1β expression via the IRAK1/TRAF6 pathway. Conclusion This study suggested that miR‐146a‐5p attenuates IL‐1β‐induced inflammatory factors in cementoblast‐derived cell line.