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In vitro adherence of Candida albicans to zirconia surfaces
Author(s) -
Zupancic Cepic Lana,
Dvorak Gabriella,
Piehslinger Eva,
Georgopoulos Apostolos
Publication year - 2020
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.13319
Subject(s) - candida albicans , adhesion , mucin , wetting , microbiology and biotechnology , saliva , chemistry , mucoadhesion , staphylococcus epidermidis , cubic zirconia , corpus albicans , biofilm , in vitro , incubation , colony forming unit , materials science , bacteria , biology , staphylococcus aureus , composite material , biochemistry , drug carrier , ceramic , drug delivery , genetics , organic chemistry
Objectives This study aimed to characterize surface properties such as roughness (Ra) and surface‐free energy (SFE) of glazed and polished yttria‐stabilized zirconia and to evaluate in vitro adherence of fungus Candida albicans and salivary bacteria, Staphylococcus epidermidis , mixed with C. albicans to these substrata. Additionally, the influence of salivary proteins (albumin, mucin and α‐amylase) on yeast adhesion was studied. Material and methods Ra and SFE of glazed and polished zirconia discs were measured. Specimens were wetted with saliva and salivary proteins prior to incubation with C. albicans and mixed suspension of C. albicans and S. epidermidis for 24 hr , respectively. Microbial adhesion was quantified by counting colony‐forming units (CFU). Differences in physicochemical properties were proved by t test. “Linear mixed model” with the factors “type of surface” and “wetting media” was applied to analyse the effects on fungal adhesion ( p < .05). Results SFE and Ra of glazed specimens were significantly higher than corresponding values of polished ones. The wetting media significantly changed the fungal binding ( p = .0016). Significantly higher quantities of adhering fungi were found after mucin incubation compared to saliva ( p = .004). For the factor “surface” as well as the interaction between “surface” and “wetting media,” no statistically significant differences have been found. In mixed suspension, the growth of Candida was completely prevented. Conclusions Glazed and polished zirconia differs in terms of physicochemical surface properties. These differences appear to be modulated by pellicle coating affecting the biomass of adhered Candida . Mucin seems to be good binding sites for adhesion of C. albicans .