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The prevalence of oral high‐risk HPV infection in Indonesian oral squamous cell carcinoma patients
Author(s) -
Purwanto Denni J.,
Soedarsono Nurtami,
Reuwpassa Jauhari O.,
Adisasmita Asri C.,
Ramli Muchlis,
Djuwita Ratna
Publication year - 2020
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.13221
Subject(s) - medicine , cancer , basal cell , population , oncology , hpv infection , nested polymerase chain reaction , polymerase chain reaction , cervical cancer , biology , gene , environmental health , genetics
Objectives The objective of this study was to report the integrated observations of high‐risk HPV‐related oral squamous carcinoma (OSCC) at our national referral center for cancer, the Dharmais National Cancer Hospital (DNCH), Jakarta, from 2003 to 2013. Materials and Methods Seventy‐eight formalin‐fixed paraffin‐embedded specimens obtained from OSCC cases were collected from 2003 to 2013 DNCH archives and were included in this high‐risk HPV (HR‐HPV) study. Seventy‐nine DNA samples from the normal oral mucosa of healthy individuals were obtained from the Oral Biology Laboratory DNA archives from 2001 to 2005. Glyceraldehyde 3‐phosphate dehydrogenase was used as a control to ensure the DNA integrity for the subsequent HPV DNA PCR detection. High‐risk HPV16/18 DNA amplification was conducted by nested PCR using two pairs of primers that were designed specifically to identify the region of gene L1 HPV16 and the HPV16/18 region. Results and Conclusions A high prevalence of HPV16/18 was detected in OSCC cases (17.9%). HPV18 occurred more often than HPV16 (86%) among OSCC patients who were HPV positive. This result supports high HPV18 prevalence among Indonesian cervical cancer patients studied in 1995 and 2006. The prevalence of high‐risk HPV remains low in the normal Indonesian population (3.8%), but HPV16 is consistently more frequently detected in non‐cancer populations.