LINE‐1 methylation in cleft lip tissues: Influence of infant  MTHFR  c.677C>T genotype | Zendy

Khan Mohammad Faisal J. | Zendy; Little Julian | Zendy; Aleotti Valentina | Zendy; Mossey Peter A. | Zendy; SteegersTheunissen Régine P. M. | Zendy; Autelitano Luca | Zendy; Meazzini Maria C. | Zendy; Ravaei Amin | Zendy; Rubini Michele | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

LINE‐1 methylation in cleft lip tissues: Influence of infant MTHFR c.677C>T genotype

Author(s) -

Khan Mohammad Faisal J.,

Little Julian,

Aleotti Valentina,

Mossey Peter A.,

SteegersTheunissen Régine P. M.,

Autelitano Luca,

Meazzini Maria C.,

Ravaei Amin,

Rubini Michele

Publication year - 2019

Publication title -

oral diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.953

H-Index - 87

eISSN - 1601-0825

pISSN - 1354-523X

DOI - 10.1111/odi.13136

Subject(s) - methylenetetrahydrofolate reductase , genotyping , genotype , methylation , dna methylation , genomic dna , allele , microbiology and biotechnology , genetics , medicine , biology , dna , gene , gene expression

Objective To investigate the influence of MTHFR c.677C>T genotype on LINE‐1 methylation in lateral and medial tissues from cleft lip (CL). Methods Forty‐five consecutive non‐syndromic cleft lip with or without cleft palate (nsCL/P) cases were included in the study. Genomic DNA was extracted from tissues at both sides of cleft lip, and LINE‐1 methylation was detected by bisulfite conversion and pyrosequencing. MTHFR c.677C>T genotyping was carried out using the TaqMan genotyping assay. Results LINE‐1 methylation level was significantly higher on medial side of cleft lip compared with lateral side ( p = 0.001). This difference was not significantly influenced by the case's sex or cleft type. However, MTHFR c.677C>T genotyping revealed that the difference in LINE‐1 methylation across cleft lip was restricted to carriers of C allele of MTHFR c.677C>T and was not apparent in TT homozygous cases ( p = 0.027). Conclusion This integrated analysis supports the previous finding of differences in DNA methylation across the two sides of cleft lip and further suggests a possible role of MTHFR c.677C>T genotype in establishing this difference.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research