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LINE‐1 methylation in cleft lip tissues: Influence of infant MTHFR c.677C>T genotype
Author(s) -
Khan Mohammad Faisal J.,
Little Julian,
Aleotti Valentina,
Mossey Peter A.,
SteegersTheunissen Régine P. M.,
Autelitano Luca,
Meazzini Maria C.,
Ravaei Amin,
Rubini Michele
Publication year - 2019
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.13136
Subject(s) - methylenetetrahydrofolate reductase , genotyping , genotype , methylation , dna methylation , genomic dna , allele , microbiology and biotechnology , genetics , medicine , biology , dna , gene , gene expression
Objective To investigate the influence of MTHFR c.677C>T genotype on LINE‐1 methylation in lateral and medial tissues from cleft lip (CL). Methods Forty‐five consecutive non‐syndromic cleft lip with or without cleft palate (nsCL/P) cases were included in the study. Genomic DNA was extracted from tissues at both sides of cleft lip, and LINE‐1 methylation was detected by bisulfite conversion and pyrosequencing. MTHFR c.677C>T genotyping was carried out using the TaqMan genotyping assay. Results LINE‐1 methylation level was significantly higher on medial side of cleft lip compared with lateral side ( p = 0.001). This difference was not significantly influenced by the case's sex or cleft type. However, MTHFR c.677C>T genotyping revealed that the difference in LINE‐1 methylation across cleft lip was restricted to carriers of C allele of MTHFR c.677C>T and was not apparent in TT homozygous cases ( p = 0.027). Conclusion This integrated analysis supports the previous finding of differences in DNA methylation across the two sides of cleft lip and further suggests a possible role of MTHFR c.677C>T genotype in establishing this difference.