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Transplantation of dental pulp‐derived cell sheets cultured on human amniotic membrane induced to differentiate into bone
Author(s) -
Takizawa Shigeta,
Yamamoto Toshiro,
Honjo KenIchi,
Sato Yoshiki,
Nakamura Koya,
Yamamoto Kenta,
Adachi Tetsuya,
Uenishi Toshihiro,
Oseko Fumishige,
Amemiya Takeshi,
Yamamoto Yoshiaki,
Kumagai Wataru,
Kita Masakazu,
Kanamura Narisato
Publication year - 2019
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.13096
Subject(s) - pulp (tooth) , dental pulp stem cells , transplantation , dentistry , dental follicle , chemistry , medicine , pathology , mesenchymal stem cell , surgery
Objective The usefulness of the amniotic membrane as a cell culture substrate has led to its use in the development of dental pulp‐derived cell sheets. We induced osteoblastic differentiation of dental pulp‐derived cell sheets and conducted histological and immunological examinations in addition to imaging assessments for regeneration of bone defects. Methods Dental pulp cells were obtained by primary culture of the dental pulp tissue harvested from extracted wisdom teeth. These cells were maintained for three to four passages. Subsequently, the dental pulp cells were seeded onto an amniotic membrane to produce dental pulp‐derived cell sheets. Following the induction of osteoblastic differentiation, the sheets were grafted into the subcutaneous tissue of the lower back and maxillary bone defect of a nude mouse. Histological and immunological examinations of both grafts were performed. Results Dental pulp‐derived cell sheets cultured on an osteoblast differentiation‐inducing medium demonstrated resemblance to dental pulp tissue and produced calcified tissue. Mineralization was maintained following grafting of the sheets. Regeneration of the maxillary bone defect was observed. Conclusion Induction of osteoblastic differentiation of the dental pulp‐derived cell sheets may be indicated for the regeneration of periodontal tissue.