Premium
Synergistic effect of hyperbaric oxygen therapy with PTH [1–34] on calvarial bone graft in irradiated rat
Author(s) -
Park KyeongMee,
Hu KyungSeok,
Choi Hyunmin,
Oh SeoEun,
Kim HyeongIl,
Park Wonse,
Kim Sungtae
Publication year - 2019
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.13037
Subject(s) - calvaria , hyperbaric oxygen , medicine , parathyroid hormone , parietal bone , urology , bone healing , calcium , endocrinology , dentistry , chemistry , surgery , skull , in vitro , biochemistry
Objective To determine the synergistic effect of parathyroid hormone (PTH) [1–34] in combination with hyperbaric oxygen (HBO) on bone graft in a rat calvarial bone defect model under impaired osteogenic conditions. Materials and Methods Twenty‐four rats were divided into three groups. Localized radiation with a single 12 Gy dose was administered to the calvaria. Four weeks after radiation, calvarial circular defects were created in the parietal bones. All defects were filled with biphasic calcium phosphate. After the bone graft, PTH [1–34] was injected subcutaneously, and HBO was administered. At 6 weeks after the bone graft, the rats were sacrificed, and specimens were harvested. Results Histomorphometric evaluation showed that the percentage of new bone area was higher in the PTH and PTH/HBO groups than in the control group. The percent residual material area was decreased in the PTH/HBO group compared with the control group. The percentage blood vessel number was highest in the PTH group. Micro‐CT evaluation showed that the new bone volume was highest in the PTH/HBO group. The residual material volume was lowest in the PTH/HBO group. Conclusion Within the limitations of this study, our data indicate that PTH combined with HBO may reverse radiation‐induced impairment of bone healing.