Premium
Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis
Author(s) -
Amaral Sérgio Antonucci,
Pereira Thaís Santos Fontes,
Brito João Artur Ricieri,
Cortelli Sheila Cavalca,
Cortelli José Roberto,
Gomez Ricardo Santigo,
Costa Fernando Oliveira,
Miranda Cota Luís Otávio
Publication year - 2019
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12994
Subject(s) - microrna , periodontitis , pathogenesis , chronic periodontitis , periodontology , microarray , gene expression , medicine , biology , gene , bioinformatics , immunology , dentistry , genetics
Abstract Objectives MicroRNAs (miRNAs) may play an important role in inflammatory response. However, the involvement of miRNAs in the pathogenesis of periodontitis is unclear. The present study aimed to compare the miRNA expression profiles in individuals with chronic (CP) or aggressive (AP) periodontitis. Materials and methods Eighteen non‐smoker individuals (CP = 9 and AP = 9) without any history of systemic diseases or previous periodontal therapies were selected at the Clinics of Periodontology from the Federal University of Minas Gerais. Gingival tissue samples were collected during the initial periodontal therapy. miRNAs were isolated, and expression patterns of 754 miRNAs were assessed with a quantitative miRNA PCR array. miRNAs expression profiles were compared between CP and AP groups. Results There were no differences observed in the miRNAs expression profiles between CP and AP ( p > 0.05). According to the microarray analyses, the most expressed miRNAs in both groups were hsa‐miR‐1274b, hsa‐let‐7b‐5p, hsa‐miR‐24‐3p, hsa‐miR‐19b‐3p, hsa‐miR‐720, hsa‐miR‐126‐3p, hsa‐miR‐17‐3p and hsa‐miR‐21‐3p. Conclusion Findings suggested no differences in miRNAs expression profiles between chronic and aggressive forms of periodontitis. The overexpression of specific miRNAs could provide insights into the pathogenesis of both forms of the disease.