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Head and neck squamous cell carcinoma drives long interspersed element‐1 hypomethylation in the peripheral blood mononuclear cells
Author(s) -
Arayataweegool Areeya,
Srisuttee Ratakorn,
Mahattanasakul Patnarin,
Tangjaturonsasme Napadon,
Kerekhanjanarong Virachai,
Kitkumthorn Nakarin,
Mutirangura Apiwat
Publication year - 2019
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12944
Subject(s) - peripheral blood mononuclear cell , head and neck squamous cell carcinoma , methylation , dna methylation , carcinogenesis , cancer research , pathology , biology , medicine , immunology , cancer , head and neck cancer , in vitro , gene expression , biochemistry , gene
Objective Alteration of long interspersed element‐1 (LINE‐1) methylation in peripheral blood mononuclear cells (PBMCs) has been simultaneously activated to breast carcinogenesis due to its secretion. To evaluate the effect in head and neck squamous cell carcinoma (HNSCC), LINE‐1 methylation levels and patterns have been measured both in vitro and in vivo. Methods Analysis of LINE‐1 methylation in cocultured models between HNSCC cell lines and normal PBMCs was performed. The observation of PBMCs of HNSCC patients compared to PBMCs of normal controls was performed using the semiquantitative combined bisulfite restriction analysis technique. Results Downregulation of LINE‐1 methylation was significantly found in the PBMCs cocultured with all HNSCC cell lines compared to normal PBMCs. Likewise, a reduction in LINE‐1 methylation levels was observed in PBMCs of HNSCC compared to normal PBMCs ( p < 0.0001). Receiver operating characteristic analysis demonstrated the potential of the unmethylated alleles ( u C u C) of LINE‐1 for distinguishing the PBMCs of HNSCC patients from normal controls with 100% sensitivity and specificity. Conclusion Our data supported that the alteration of LINE‐1 methylation levels in PBMCs was influenced by HNSCC secretions. Moreover, the unmethylated LINE‐1 allele of PBMCs was proved to be an effective tumor marker and possesses a potential as HNSCC diagnostic tool.