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Cross‐contamination of the human salivary gland HSG cell line with HeLa cells: A STR analysis study
Author(s) -
Lin LiChieh,
Elkashty Osama,
Ramamoorthi Murali,
Trinh Nathalie,
Liu Younan,
SunavalaDossabhoy Gulshan,
Pranzatelli Thomas,
Michael Drew G.,
Chivasso Clara,
Perret Jason,
Chiorini John A.,
Delporte Christine,
Tran Simon D.
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12920
Subject(s) - hela , cell culture , saliva , biology , microbiology and biotechnology , andrology , chemistry , medicine , genetics , biochemistry
Objectives The human salivary gland ( HSG ) cell line, labeled as a submandibular ductal cell line, is commonly used as in vitro models to study radiation therapy, Sjögren's syndrome, pleomorphic adenoma, mucocele, epithelial‐to‐mesenchymal transition, and epigenetics. However, the American Type Culture Collection ( ATCC ) has recently released a list of cross‐contaminated cell lines that included HSG . Despite this notice, some research laboratories still use HSG as a salivary cell model. Therefore, this study examined the authenticity of HSG sampled from three different laboratories. Methods DNA was extracted from HSG and additional salivary cell lines ( NS ‐ SV ‐ AC , NS ‐ SV ‐ DC , A253, HSY ) and submitted for cell line authentication with short tandem repeat ( STR ) analysis. Results All HSG samples had STR profiles indicating >80% match with HeLa in both the ATCC and Deutsche Sammlung von Mikroorganismen und Zellkulturen ( DSMZ ) databases. This confirmed that HSG sampled from three different laboratories and HSY shared a common ancestry (host) with HeLa, whereas NS ‐ SV ‐ AC , NS ‐ SV ‐ DC , and A253 had unique STR profiles. Conclusion Short tandem repeat analysis revealed that HSG was contaminated by the HeLa cell line. Furthermore, because genotyping of the original HSG cell line was not performed during its establishment, it will be difficult to authenticate an uncontaminated sample of HSG .