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Stress‐related salivary proteins affect the production of volatile sulfur compounds by oral bacteria
Author(s) -
Lima Patricia Oliveira,
Nani Bruno Dias,
Almeida Barbara,
Marcondes Fernanda Klein,
Groppo Francisco Carlos,
de Moraes Antonio Bento Alves,
FranzMontan Michelle,
CogoMüller Karina
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12890
Subject(s) - affect (linguistics) , bacteria , sulfur , salivary proteins , microbiology and biotechnology , chemistry , medicine , biochemistry , saliva , biology , psychology , organic chemistry , genetics , communication
Objective To determine whether stress‐related substances and sex hormones influence the growth and in vitro production of volatile sulfur compounds ( VSC s) by Solobacterium moorei and Fusobacterium nucleatum . Materials and Methods Bacteria growth and VSC s production were evaluated in the presence of alpha‐amylase, beta‐defensin‐2, mucin, estradiol, and progesterone. Growth was evaluated by colony counting, and the production of the VSC s hydrogen sulfide (H 2 S) and methyl mercaptan ( CH 3 SH ) was measured using the Oral Chroma™ instrument. Results Mucin induced the production of H 2 S by both bacteria, but had a slight inhibitory effect on CH 3 SH production by F. nucleatum . It also increased the viability of F. nucleatum . Alpha‐amylase increased H 2 S production by S. moorei and CH 3 SH production by F. nucleatum , but had no effect on H 2 S production by F. nucleatum . No substance altered the viability of S. moorei . No effects of beta‐defensin‐2, estradiol, or progesterone were observed. Conclusion The salivary stress‐related proteins mucin and alpha‐amylase altered VSC s production by F. nucleatum and S. moorei, favoring H 2 S production. These findings are a step toward understanding the relation between stress and increased amounts of H 2 S.