Premium
Image cytometry DNA ploidy analysis: Correlation between two semi‐automated methods
Author(s) -
Ghizoni Janaina S.,
Sperandio Marcelo,
Lock Claire,
Odell Edward W.
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12888
Subject(s) - ploidy , wilcoxon signed rank test , cytometry , concordance , biology , flow cytometry , microbiology and biotechnology , genetics , medicine , gene , mann–whitney u test
Background and Objectives Quantitation of cell DNA content, DNA ploidy, has been established as a research and prognostic technique for decades. A variety of instruments have been used although only a few commercially available systems have established quality assurance and published outcome data. The aim of this study was to compare two automated systems. Methods Nuclear monolayers were obtained from 112 oral biopsies by enzyme digestion and Feulgen staining. These were scanned on both the Fairfield and the Ploidy Work Station ( PWS ) systems. The overall ploidy diagnosis, number of epithelial nuclei, coefficient of variation ( CV ) and 5c exceeding rate (5 CER ) were compared by quantile–quantile plots, t test, Wilcoxon and Spearman's tests. Results The PWS system identified more nuclei ( p < 0.0001) at a lower CV ( p < 0.0001). Using the PWS system, fewer samples were classified as indeterminate. No difference between 5 CER was found between systems ( p > 0.54). There was complete concordance between the two systems in terms of DNA ploidy diagnosis. Conclusions The PWS system is comparable to the Fairfield system for determination of DNA ploidy and has advantages that may lead to improved performance.