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Effects of BMP ‐2 on the osteogenic differentiation of bone marrow stem cells in fibrous dysplasia
Author(s) -
Um S,
Kim HY,
Seo BM
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12869
Subject(s) - osteocalcin , in vivo , chemistry , homeobox protein nanog , stem cell , bone marrow , bone morphogenetic protein 2 , pathology , bone marrow stem cell , transplantation , staining , alkaline phosphatase , in vitro , microbiology and biotechnology , medicine , biology , embryonic stem cell , induced pluripotent stem cell , biochemistry , gene , enzyme
Objectives The purpose of this study was to evaluate the effects of BMP ‐2 on bone‐grafting procedures for the treatment of fibrous dysplasia based on in vitro and in vivo experiments. Subjects and Methods Proliferation of stem cells was determined by colony‐forming assay, CCK ‐8 assay and BrdU staining. OCT 4 and NANOG expression was analysed by flow cytometry and immunocytochemistry. Osteogenic differentiation was assessed by measuring ALP activity, Alizarin red S staining and in vivo transplantation. Gene expression of the osteogenic markers, osteocalcin and type 1 collagen, was determined by RT ‐ PCR . Results FD ‐ BMSC s showed few calcium deposits and low ALP activity. Bone formation by transplanted FD ‐ BMSC s was also suppressed relative to that of normal BMSC s. However, BMP ‐2 treatment enhanced osteogenic differentiation of FD ‐ BMSC s mixed with normal BMSC s in vitro and in vivo. Overall, BMP ‐2 treatment promoted osteogenic differentiation of FD ‐ BMSC s mixed with normal BMSC s. Conclusions In patients with FD , stem cells in affected bone are influenced by the mutation, resulting in weak bone formation with the proliferation of immature osteogenic cells. Current treatment of FD involves surgical removal of excess bulk lesions, which can cause facial disfigurement. Our results suggest that BMP ‐2 application is a good adjunctive modality to the surgical treatment of patients with FD .