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Saliva with reduced calcium and phosphorous concentrations: Effect on erosion dental lesions
Author(s) -
Denucci GC,
Mantilla TF,
Amaral FLB,
Basting RT,
França FMG,
Turssi CP
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12843
Subject(s) - enamel paint , saliva , knoop hardness test , calcium , chemistry , dentistry , hydrochloric acid , molar , nuclear chemistry , indentation hardness , medicine , biochemistry , inorganic chemistry , microstructure , organic chemistry , crystallography
Objectives To investigate whether saliva formulations with reduced calcium (Ca) and inorganic phosphorous (Pi) concentration would affect dental erosion caused by hydrochloric acid (HCl). Methods Enamel and root dentine bovine slabs were embedded, polished, and measured for surface Knoop microhardness (SMH). After reference areas were created, specimens were exposed to HCl solution (0.01 M; pH 2; 120 s) and immersed in artificial salivas (6 hr) containing three different Ca/Pi concentrations ( n = 15), which simulate serum conditions of normo‐, mild, or severe hypocalcemia. The control group was immersed in Ca/Pi‐free saliva. The study protocol was carried out 2×/day for 5 days. Surface loss of enamel and root dentine was assessed using an optical profilometer, and SMH was remeasured for enamel. Results One‐way analysis of variance ( p < .001) and Tukey’s test showed that enamel loss in groups subjected to artificial salivas that simulated mild or severe hypocalcemia did not differ from that resembling normocalcemia. %SMH was lower when saliva was mildly and normally concentrated in Ca/Pi ( p < .001). Root dentine loss was higher in saliva simulating severe hypocalcemia than in those referring to mild, hypo‐, and normocalcemia. Conclusions Depending on the dental substrate, salivary formulations resembling serum hypocalcemia affected surface loss due to erosion and rehardening thereof.