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mi RNA expression profile of mucoepidermoid carcinoma
Author(s) -
Binmadi NO,
Basile JR,
Perez P,
Gallo A.,
Tandon M,
Elias W,
Jang SI,
Alevizos I
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12800
Subject(s) - microrna , mucoepidermoid carcinoma , downregulation and upregulation , malignancy , taqman , biology , cancer research , carcinoma , real time polymerase chain reaction , pathology , medicine , gene , genetics
Objectives Micro RNA s (mi RNA s) are single‐stranded RNA s that have been implicated in cancer initiation and progression and act as tumour suppressors or oncogenes. In this study, mi RNA profiling was conducted on the most frequent malignancy of salivary glands, mucoepidermoid carcinoma ( MEC ), in comparison with normal tissues. Materials and Methods The TaqMan Human mi RNA Cards Array was used for the mi RNA profiling of MEC and normal tissues. To validate the differentially expressed mi RNA s in MEC , we used real‐time PCR ( qRT ‐ PCR ). Results miR‐302a was the most significantly increased mi RNA in cancer tissues ( p < .05). Here, we demonstrate that the upregulation of miR‐302a expression in SGT cell lines induced cancer cell invasion in vitro. Conclusions These promising results suggest the need for further studies to establish mir‐302a as a marker of invasion and aggressiveness in MEC .