Premium
Plumbagin‐mediating GLUT 1 suppresses the growth of human tongue squamous cell carcinoma
Author(s) -
Na S,
Zhang J,
Zhou X,
Tang A,
Huang D,
Xu Q,
Xue D,
Qiu J
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12799
Subject(s) - glut1 , pi3k/akt/mtor pathway , downregulation and upregulation , plumbagin , cancer research , protein kinase b , in vivo , cell growth , chemistry , biology , apoptosis , glucose transporter , endocrinology , biochemistry , genetics , microbiology and biotechnology , gene , insulin
Objective The purpose of this study was to investigate the clinical and histopathological characteristics of GLUT 1 in human tongue squamous cell carcinoma ( TSCC ) and the role of plumbagin ( PLB )‐mediating GLUT 1 in the growth of TSCC . Subjects and methods Forty‐five cases of TSCC samples were collected and the expression and location of GLUT 1 was analyzed. The role and mechanism of PLB meditating GLUT 1 in the inhibitory growth of human TSCC cell line CAL 27 were investigated in vitro and vivo . Results The expression of GLUT 1 was observed in all samples of human TSCC by immunohistochemical staining. GLUT 1 expression was significantly correlated with lymph node metastasis and clinical stage in TSCC . PLB treatment decreased cell viability and colony formation, and increased cell apoptosis in association with the downregulation of GLUT 1 via inhibiting PI 3K/Akt pathway in vitro and PLB suppressed tumor growth in correlation with downregulation of GLUT 1, compared with control group in vivo. Conclusions The findings demonstrated a novel anti‐cancer mechanism of PLB , inhibitory TSCC growth via suppressing PI 3K/Akt/ GLUT 1 pathway, which will supply a theoretical basis for PLB to treat TSCC .