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Low‐intensity pulsed ultrasound stimulation for mandibular condyle osteoarthritis lesions in rats
Author(s) -
Kanaguchi Arita A,
Yonemitsu I,
Ikeda Y,
Miyazaki M,
Ono T
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12798
Subject(s) - low intensity pulsed ultrasound , ultrasound , osteoarthritis , intensity (physics) , medicine , cartilage , immunohistochemistry , temporomandibular joint , condyle , bone mineral , chemistry , pathology , anatomy , nuclear medicine , therapeutic ultrasound , radiology , osteoporosis , physics , alternative medicine , quantum mechanics
Objective This study evaluated low‐intensity pulsed ultrasound effects for temporomandibular joint osteoarthritis in adult rats. Material and methods Osteoarthritis‐like lesions were induced in 24 adult rats’ temporomandibular joints with low‐dose mono‐iodoacetate injections. The rats were divided into four groups: control and mono‐iodoacetate groups, injected with contrast media and mono‐iodoacetate, respectively, at 12 weeks and observed until 20 weeks; and low‐intensity pulsed ultrasound and mono‐iodoacetate + low‐intensity pulsed ultrasound groups, injected with contrast media and mono‐iodoacetate, respectively, at 12 weeks with low‐intensity pulsed ultrasound performed from 16 to 20 weeks. Condylar bone mineral density, bone mineral content and bone volume were evaluated weekly with microcomputed tomography. Histological and immunohistochemical staining for matrix metalloproteinases‐13 was performed at 20 weeks. Results At 20 weeks, the mono‐iodoacetate + low‐intensity pulsed ultrasound group showed significantly higher bone mineral density, bone mineral content and bone volume than the mono‐iodoacetate group; however, these values remained lower than those in the other two groups. On histological and immunohistochemical analysis, the chondrocytes were increased, and fewer matrix metalloproteinases‐13 immunopositive cells were identified in the mono‐iodoacetate + low‐intensity pulsed ultrasound group than mono‐iodoacetate group. Conclusions Low‐intensity pulsed ultrasound for 2 weeks may have therapeutic potential for treating temporomandibular joint osteoarthritis lesions.