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Harmine promotes periodontal ligament cell‐induced tissue regeneration
Author(s) -
Lim HC,
Cha BY,
Song SU,
Yun JH
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12770
Subject(s) - harmine , chemistry , flow cytometry , blot , microbiology and biotechnology , biophysics , biochemistry , biology , pharmacology , gene
Objective to investigate whether harmine has a promotive effect on human periodontal ligament cells ( hPDLC s)‐induced tissue regeneration. Materials and Methods Various concentrations of harmine on hPDLC s proliferation were tested. Osteogenic and cementogenic characteristics were examined in hPDLC /rh BMP ‐2 and hPDLC /harmine by alizarin red S staining, real‐time PCR , and Western blotting assay. The activity of harmine was investigated in an ectopic transplantation nude mouse model. Results We determined that 10 μM of harmine was the threshold concentration. hPDLC /harmine showed similar mineralized nodule formation in alizarin S staining compared to hPDLC /rh BMP ‐2. In real‐time PCR , the highest gene expression level was observed for Runx2 in hPDLC /harmine at all time points. The level of CEMP ‐1 in hPDLC /harmine was higher at 7 days than hPDLC s alone. Thicker band of Runx2 in hPDLC /harmine was observed than in hPDLC /rh BMP ‐2 at 7 days by Western blotting. The band for CEMP ‐1 in hPDLC /harmine was thicker than hPDLC s alone at both 7 and 14 days. In ectopic transplantation, hPDLC s with harmine showed a comparable amount of mineralized tissue formation compared to rh BMP ‐2. hPDLC s with harmine or rh BMP ‐2 formed both bone and cementum‐like tissue with Sharpey's fiber‐like collagen insertion. Conclusion Harmine can be a potential candidate for promoting hPDLC s‐induced tissue regeneration.