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Human papillomavirus load in benign HPV ‐associated oral lesions from HIV / AIDS individuals
Author(s) -
CamachoAguilar S,
RamírezAmador V,
RosendoChalma P,
GuidoJiménez M,
GarcíaCarrancá A,
AnayaSaavedra G
Publication year - 2018
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12732
Subject(s) - human papillomavirus , medicine
Background Although HPV emerged as a crucial carcinogenic and prognostic biomarker in head and neck cancer, and considering the increase in HPV ‐associated oral lesions ( HPV ‐ OL s) in HIV individuals, molecular information about HPV ‐ OL s is scarce; thus, our aim was to determine viral loads in HPV ‐ OL s from HIV / AIDS individuals. Methods HIV / AIDS subjects with HPV ‐ OL were included in this cross‐sectional study. Following informed consent, biopsies were obtained. HPV detection and typing were carried out by PCR and sequencing ( MY 09/11, GP 5+/6+). HPV ‐13 and HPV ‐32 loads were determined by a high‐resolution melting assay. For statistical analysis, X 2 , Fisher's exact, and Mann‐Whitney U tests were applied, using SPSS software (v.23). Results Twenty‐nine HIV subjects (median age 38 years, 93% males) were included. Most were AIDS individuals (72.4%) under HAART (89.7%). Twenty‐two (75.9%) participants had more than one HPV ‐ OL (four with florid presentations), mostly multifocal epithelial hyperplasia (62%), being HPV ‐13 (26%) and HPV ‐32 (31%) the most frequent types. HPV load was higher in individuals with multiple HPV ‐ OL s than in solitary lesions (4.9 vs. 3.2 Log 10 copies/ ml , p = .090) and in HPV ‐32 + than in HPV ‐13 + (8.3 vs. 6.4 Log 10 copies/ ml , p = .014). Conclusions Multiple HPV ‐ OL s showed high HPV loads, possibly indicating transcriptional activity of the virus; however, in the HIV setting, the individual and local immunological response could be the key process.