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Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus
Author(s) -
Portilho MM,
Mendonça ACF,
Marques VA,
Nabuco LC,
VillelaNogueira CA,
Ivantes CAP,
LewisXimenez LL,
Lampe E,
Villar LM
Publication year - 2017
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12692
Subject(s) - viral load , concordance , virology , hepatitis b virus , medicine , hepatitis b , polymerase chain reaction , microbiology and biotechnology , biology , virus , biochemistry , gene
Background This study aims to compare the efficiency of four oral fluid collection methods (Salivette, FTA Card, spitting and DNA ‐Sal) to detect HBV DNA by qualitative PCR . Materials and Methods Seventy‐four individuals (32 HBV reactive and 42 with no HBV markers) donated serum and oral fluid. In‐house qualitative PCR to detect HBV was used for both samples and commercial quantitative PCR for serum. Results HBV DNA was detected in all serum samples from HBV ‐infected individuals, and it was not detected in control group. HBV DNA from HBV group was detected in 17 samples collected with Salivette device, 16 samples collected by FTA Card device, 16 samples collected from spitting and 13 samples collected by DNA ‐Sal device. Samples that corresponded to a higher viral load in their paired serum sample could be detected using all oral fluid collection methods, but Salivette collection device yielded the largest numbers of positive samples and had a wide range of viral load that was detected. Conclusion It was possible to detect HBV DNA using all devices tested, but higher number of positive samples was observed when samples were collected using Salivette device, which shows high concordance to viral load observed in the paired serum samples.