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Experimental study on 1,25( OH ) 2 D 3 amelioration of oral lichen planus through regulating NF‐ κ B signaling pathway
Author(s) -
Du J,
Li R,
Yu F,
Yang F,
Wang J,
Chen Q,
Wang X,
Zhao B,
Zhang F
Publication year - 2017
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12659
Subject(s) - hacat , calcitriol receptor , nf κb , signal transduction , chemistry , blot , lipopolysaccharide , proinflammatory cytokine , downregulation and upregulation , nfkb1 , cancer research , microbiology and biotechnology , receptor , inflammation , biology , endocrinology , immunology , transcription factor , biochemistry , in vitro , gene
Objective To explore the protective function of vitamin D ( VD )/vitamin D receptor ( VDR ) on the development of oral lichen planus ( OLP ) and elaborate the underling mechanism of it. Methods H&E staining, myeloid peroxidase ( MPO ) assays, quantitative PCR ( qPCR ), Western blotting, and Elisa were used to test the human biopsies and serum. QPCR , Western blotting, Elisa, and si RNA transfection were also performed in LPS ‐induced keratinocytes to observe the functions of vitamin D and VDR . Results The lack of VDR in the diseased biopsies from OLP patients was associated with activated helper T‐cell type 1 (Th1)‐driven inflammatory response. Importantly, the status of serum 25‐hydroxyvitamin D of OLP patients was reduced consistently. In a cultured cell model, 1,25( OH ) 2 D 3 could downregulate excessive production of pro‐inflammatory factors induced by lipopolysaccharide ( LPS ) in keratinocyte HaCat cells. Mechanistically, even though LPS ‐induced cytokines in keratinocytes were inhibited both by nuclear factor‐ κ B ( NF ‐ κ B) inhibitor and by activator protein 1 ( AP ‐1) inhibitor, VDR ‐dependent 1,25( OH ) 2 D 3 blocked the activation of phosphorylated‐ NF ‐ κ B p65 rather than c‐Jun/c‐Fos in the presence of LPS stimulation. Conclusion These results suggest that 1,25( OH ) 2 D 3 plays an anti‐inflammatory role in OLP by mediating NF ‐ κ B signaling pathway but not AP ‐1 signaling pathway with a VDR ‐dependent manner, predicting vitamin D supplement may be a potential strategy for the OLP management.