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Absence of the frequently reported PIK 3 CA , CASP 8, and NOTCH 1 mutations in South Indian oral cancers
Author(s) -
Arunkumar G,
Murugan AK,
Nagarajan M,
Ajay C,
Rajaraman R,
Munirajan AK
Publication year - 2017
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12655
Subject(s) - mutation , chemistry , microbiology and biotechnology , biology , genetics , gene
Objectives Somatic mutations of the PIK 3 CA , CASP 8, and NOTCH 1 have been frequently detected in various human cancers. Our study aimed to analyze the mutational status of these genes in South Indian oral cancers. Subjects and methods We performed mutational analysis of the PIK 3 CA (exons 9 and 20), CASP 8 (exon 9), and NOTCH 1 (exons 5, 6, 7, 8, and 9) genes in 96, 48, and 44 oral cancer samples, respectively. All the specified exons were PCR (polymerase chain reaction)‐amplified and directly sequenced by Sanger sequencing. Results PIK 3 CA gene mutations were not found; however, a synonymous single nucleotide polymorphism ( SNP ) [rs17849079] was observed frequently [35/96 (36.4%)] in oral cancer samples. Further, no mutations were detected in the CASP 8 gene, but observed a frequent [32/48 (66.6%)] SNP [rs1045487] in the oral cancer samples. We did not detect any mutation in the NOTCH 1 gene (exons 5, 6, 7, 8, and 9) in all the [0/44] analyzed oral cancer samples. Conclusions This is the first study that reports the status of the PIK 3 CA , CASP 8, and NOTCH 1 mutations in South Indian oral cancer samples. Our study suggests that either mutations in these genes are uncommon in South Indian oral cancer samples or likely other genes in this pathway might be mutated.