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Immunoexpression of HDAC 1, HDAC 2, and HAT 1 in actinic cheilitis and lip squamous cell carcinoma
Author(s) -
Chrun ES,
Modolo F,
Vieira DSC,
BorgesJúnior ÁLS,
Castro RG,
Daniel FI
Publication year - 2017
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12641
Subject(s) - acetylation , histone acetyltransferases , chemistry , histone , cancer research , immunostaining , cell , immunohistochemistry , microbiology and biotechnology , biology , gene , biochemistry , immunology
Background Acetylation and deacetylation are the most studied covalent histone modifications resulting in transcriptional regulation with histone deacetylases ( HDAC ) and histone acetyltransferases ( HAT ) as the main associated enzymes. These enzymes overexpression induces abnormal transcription of key genes that regulate important cellular functions, such as proliferation, cell cycle regulation, and apoptosis. Thus, the expression of different HAT s and HDAC s has been evaluated in various cancers. Objective To investigate HDAC 1, HDAC 2 and HAT 1 expression in lip squamous cell carcinoma ( LSCC ) and actinic cheilitis ( AC ) and to demonstrate their correlation with DNA metyltransferases ( DNMT s). Material and methods Thirty cases of lip squamous cell carcinoma ( LSCC ), thirty cases of actinic cheilitis ( AC ), and 28 cases of non‐neoplastic epithelium as control were selected for immunohistochemical investigation. Results Nuclear HDAC 2 immunopositivity was significantly higher in AC (75.07% ± 29.70) when compared with LSCC (51.06% ± 39.02). HDAC 1 and HAT 1 nuclear immunostaining were higher in AC , with no statistical significance. When comparing data with our previous study, we found a positive correlation between HDAC 1 X DNMT 1/ DNMT 3b, HDAC 2 X DNMT 3b, and HAT 1 X DNMT 1/ DNMT 3b for certain studied groups. Conclusion This study showed higher levels of nuclear HDAC 2 immunopositivity in AC , possibly indicating that this enzyme plays a key role in lip photocarcinogenesis early stages.