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Novel reuterin‐related compounds suppress odour by periodontopathic bacteria
Author(s) -
Fujiwara N,
Murakami K,
Nakao M,
Toguchi M,
Yumoto H,
Amoh T,
Hirota K,
Matsuo T,
Sano S,
Ozaki K,
Miyake Y
Publication year - 2017
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12638
Subject(s) - fusobacterium nucleatum , porphyromonas gingivalis , bacteria , chemistry , in vitro , microbiology and biotechnology , bacteroidaceae , biology , biochemistry , genetics
Objective Halitosis is caused by volatile sulphur compounds including methyl mercaptan ( CH 3 SH ) in the oral cavity and is a serious problem that limits interpersonal social communication. The aim of study was to evaluate the effects of reuterin‐related compounds ( RRC s) on halitosis‐related periodontopathic bacteria in vitro . Materials and Methods RRC ‐01, RRC ‐02 and RRC ‐03 (32 and 64 μ g ml −1 ) in culture media containing Fusobacterium nucleatum JCM 8523 and Porphyromonas gingivalis ATCC 33277 were used. The effects of RRC s on CH 3 SH production and detectable odour by F. nucleatum and P. gingivalis were examined by CH 3 SH production assay and organoleptic test, respectively. The number of bacterial cells was also measured using an ATP assay. In P. gingivalis treated with RRC s, the expression of mgl gene, which is responsible for CH 3 SH production, was examined by q RT ‐PCR. Results CH 3 SH production and the score of detectable odour from F. nucleatum and P. gingivalis culture media containing RRC s were significantly lower than that without RRC s ( P < 0.05). The expression of mgl gene in P. gingivalis was significantly downregulated by RRC ‐01 ( P < 0.01), but not by RRC ‐02 or RRC ‐03. Conclusions RRC s are potent oral care products for preventing halitosis via reducing CH 3 SH production.