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Allelic loss in amalgam‐associated oral lichenoid lesions compared to oral lichen planus and mucosa
Author(s) -
Rodrigues LN,
Sousa SF,
Silva RCC,
Abreu MHG,
Pires FR,
Mesquita RA,
BastosRodrigues L,
De Marco L,
Gomes CC,
Gomez RS,
Bernardes VF
Publication year - 2017
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12627
Subject(s) - loss of heterozygosity , oral lichen planus , pathology , oral mucosa , pathogenesis , immunohistochemistry , microsatellite , proliferative index , ki 67 , biology , lesion , medicine , allele , genetics , gene
Background The amalgam‐associated oral lichenoid lesion ( AAOLL ) shows clinical and histopathological features similar to oral lichen planus ( OLP ). Molecular researches to improve knowledge of pathogenesis and clinical behavior of AAOLL are still scarce. Objective We investigated for the first time the use of loss of heterozygosity ( LOH ) as a molecular approach for genetic characterization of AAOLL in comparison with OLP and evaluated the cell proliferation index. Materials and Methods The sample comprised nine AAOLL s, 10 OLP s, and eight NOM s matched by patients’ gender and age. LOH was assessed using polymorphic microsatellite markers at chromosomes 9p (D9S157, D9S162, D9S171), 11q (D11S1369), and 17p ( TP 53, AFM 238 WF 2). Cell proliferation was assessed by immunohistochemical expression of Ki‐67 ( MIB ‐1). The association between LOH and Ki‐67 was investigated. Results Loss of heterozygosity occurred in 5/9 AAOLL s and in 2/10 OLP s in at least one marker each, while NOM showed no LOH . Cell proliferation index in AAOLL ranged from 2 to 23%. There was no association between cell proliferation and LOH , independent of the marker. Conclusion Our study shows that the profile of molecular changes in AAOLL and OLP , evaluated by LOH and Ki‐67 expression, is similar. Additional studies including larger samples should be performed to confirm or to refute our findings.