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SATB 1 promotes tumor metastasis and invasiveness in oral squamous cell carcinoma
Author(s) -
Li YC,
Bu LL,
Mao L.,
Ma SR,
Liu JF,
Yu GT,
Deng WW,
Zhang WF,
Sun ZJ
Publication year - 2017
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12602
Subject(s) - gene knockdown , metastasis , epithelial–mesenchymal transition , cancer research , immunohistochemistry , biology , motility , tissue microarray , pathology , cell , cell culture , cancer , medicine , immunology , microbiology and biotechnology , genetics
Objective Our aim is to evaluate the expression of SATB 1 in human oral squamous cell carcinomas ( OSCC ) and its role in the invasiveness and metastasis of OSCC . Subjects and methods A human OSCC tissue microarray was used to evaluate the expression pattern of SATB 1. SATB 1 mRNA knockdown was performed in human OSCC cell lines SCC 25 and Cal27 to assess the function of SATB 1 in the invasiveness and metastasis of OSCC . Results SATB 1 is highly expressed in human OSCC determined by immunohistochemistry, and its nuclear/cytoplasmic ratio of histoscore is significantly correlated with patients’ prognosis. Reduced cell motility, invasiveness, expression of epithelial to mesenchymal transition ( EMT ) markers (N‐cadherin and β‐catenin), and elevated expression of epithelial markers were observed in SATB 1‐knockdown cells in in vitro studies. Depletion of SATB 1 also restored a cobblestone‐like morphology in TGF ‐β1‐treated cells. Conclusions These findings suggest SATB 1 may play an important role in OSCC invasiveness and metastasis.
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