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CD 36 is upregulated in mice with periodontitis and metabolic syndrome and involved in macrophage gene upregulation by palmitate
Author(s) -
Lu Z,
Li Y,
Brinson CW,
Kirkwood KL,
LopesVirella MF,
Huang Y
Publication year - 2017
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12596
Subject(s) - periodontitis , downregulation and upregulation , endocrinology , medicine , lipopolysaccharide , cd36 , inflammation , porphyromonas gingivalis , gene expression , chemistry , receptor , gene , biochemistry
Background We reported that high‐fat diet ( HFD )‐induced metabolic syndrome (MetS) exacerbates lipopolysaccharide ( LPS )‐stimulated periodontitis and palmitate, the major saturated fatty acid in the HFD , amplified LPS ‐stimulated gene expression in vitro . As CD 36 is a major receptor for fatty acids, we investigated periodontal CD 36 expression in mice with periodontitis and MetS, and the role of CD36 in inflammatory gene expression in macrophages stimulated by palmitate. Methods MetS and periodontitis were induced in mice by HFD and periodontal injection of LPS , respectively. The periodontal CD 36 expression and its relationship with alveolar bone loss were studied using immunohistochemistry, real‐time PCR , and correlation analysis. The role of CD 36 in upregulation of inflammatory mediators by LPS and palmitate in macrophages was assessed using pharmacological inhibitor and small interfering RNA . Results Periodontal CD 36 expression was higher in mice with both MetS and periodontitis than that in mice with periodontitis or MetS alone and was correlated with osteoclastogenesis and alveolar bone loss. In vitro studies showed that CD 36 expression in macrophages was upregulated by LPS and palmitate, and targeting CD 36 attenuated palmitate‐enhanced gene expression. Conclusion CD 36 expression is upregulated in mice with periodontitis and MetS and involved in gene expression in macrophages stimulated by palmitate and LPS .