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In vivo photodynamic inactivation of Candida albicans using chloro‐aluminum phthalocyanine
Author(s) -
Carmello JC,
Alves F,
Ribeiro APD,
Basso FG,
Souza Costa CA,
Tedesco AC,
Primo FL,
Mima EG,
Pavarina AC
Publication year - 2016
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12466
Subject(s) - candida albicans , photosensitizer , corpus albicans , photodynamic therapy , in vivo , tongue , phthalocyanine , chemistry , medicine , pathology , microbiology and biotechnology , biology , photochemistry , organic chemistry
This study evaluated the photoinactivation of Candida albicans in a murine model of oral candidiasis using chloro‐aluminum phthalocyanine (ClAlP) encapsulated in cationic nanoemulsions ( NE ) and chloro‐aluminum phthalocyanine (ClAlP) diluted in DMSO ( DMSO ) as photosensitizer ( PS ). Seventy‐five 6‐week‐old female Swiss mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDT was performed on the tongue by the application of the photosensitizers and LED light (100 J cm −2 –660 nm). Twenty‐four hours and 7 days after treatments, microbiological evaluation was carried out by recovering C. albicans from the tongue of animals (CFU ml −1 ). Then, mice were sacrificed and the tongues were surgically removed for histological and biomolecular analysis of pro‐ and anti‐inflammatory cytokines. Data were analyzed by ANOVA followed by Tukey's post hoc test. ClAlP‐ NE ‐mediated PDT reduced 2.26 log 10 of C. albicans recovered from the tongue when compared with the control group (P−L−) ( P < 0.05). PDT did not promote adverse effects on the tongue tissue. Seven days after treatment, all animals were completely healthy. In summary, PDT mediated by chloro‐aluminum phthalocyanine entrapped in cationic nanoemulsions was effective in reducing C. albicans recovered from the oral lesions of immunocompromised mice.