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Diagnostic profiling of salivary exosomal micro RNA s in oral lichen planus patients
Author(s) -
Byun JS,
Hong SH,
Choi JK,
Jung JK,
Lee HJ
Publication year - 2015
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12374
Subject(s) - oral lichen planus , saliva , microvesicles , pathogenesis , microrna , biomarker , microarray , medicine , disease , exosome , immunology , pathology , biology , gene , gene expression , genetics
Objective Oral lichen planus is a chronic inflammatory oral mucosal disease whose exact cause is unclear and which requires efficient diagnostic and therapeutic strategies. Identification of disease‐specific biomarkers in saliva is an easy, quick, and non‐invasive approach for molecular diagnosis. This study was designed to examine salivary exosomal micro RNA s (mi RNA s) that could be candidates for diagnosing and elucidating the pathogenesis of oral lichen planus. Subjects and Methods We compared mi RNA profiles of salivary exosomes of patients with oral lichen planus with those of healthy controls. Saliva samples from 16 patients with oral lichen planus and eight healthy controls were divided into two sets and examined using mi RNA microarray analysis and TaqMan quantitative PCR . Results The three mi RNA s identified (miR‐4484, miR‐1246, and miR‐1290) were further validated. Of these, miR‐4484 was significantly upregulated in the salivary exosomes of patients with oral lichen planus. Conclusions This study thus identifies a potential mi RNA biomarker for oral lichen planus and provides insight into the functions of mi RNA s in the pathogenesis of oral inflammatory diseases.